CD52 anticorps

N° du produit ABIN7538775

Cet anticorps anti-CD52 est un anticorps Souris Monoclonal détectant CD52 dans FACS et IHC. Adapté pour Humain.

Aperçu rapide pour CD52 anticorps (ABIN7538775)

Antigène: CD52

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD52 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: HI186

Détail du produit anti-CD52 anticorps

Specificité: The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29 kDa.

Réactivité croisée (Details): No reaction was observed against Human, Mouse and Goat Serum Proteins.

Attributs du produit: Purifieded Mouse Monoclonal Anti-Human CD52 is recommended for use in flow cytometry for identification of CAMPATH-1 protein expressed on thymocytes, lymphocytes, monocytes and macrophagues.

Purification: Purified

Stérilité: 0.22 μm filtered

Immunogène: Human Peripheral Blood Mononuclear Cells and Tonsil cells

Isotype: IgG2b kappa

Information d'application

Indications d'application: It is recommended for use in flow cytometry. This reagent is effective for indirect immunofluorescence staining of human tissue for flow cytometric analysis using 10 μl/10^6 cells.

Commentaires:

Purifieded Mouse Monoclonal Anti-Human CD52 is recommended for use in flow cytometry for identification of CAMPATH-1 protein expressed on thymocytes, lymphocytes, monocytes and macrophagues.

Procédure de l'essai:

Indirect cell surface Protocol:

1. Add 10 µL of CD52 PU to a 12 x 75 mm polystyrene test tube. The 10 µL is a guideline only; the optimal volume should be determined by the individual laboratory
2. Transfer 100 µL of anticoagulated (EDTA) blood or the study sample (106 cells) and mix gently with a vortex mixer.
3. Incubate in the dark at room temperature (20-25 °C) for 15 minutes or at 4 °C for 30 minutes.
4. Add 2 mL 0.01 mol/L PBS and resuspend the cells. Mix well.
5. Centrifuge at 540xg for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µL of fluid.
6. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.1 mL PBS
7. Add conjugated antibody Anti-Mouse and mix gently with a vortex mixer. Incubate in the dark at room temperature (20-25 °C) for 15 minutes or at 4 °C for 30 minutes.
8. Add Lysing Solution according to the manufacturer's directions to each sample and mix gently with a vortex mixer.
9. Centrifuge at 540xg for 5 minutes. Gently aspirate the supernatant without disturbing the cell pellet and discard it leaving approximately 50 µL of fluid.
10. Add 2 mL 0.01 mol/L PBS (It better that it containing 0,5 % bovine serum albumin) and resuspend the cells. Mix well.
11. Centrifuge at 540xg for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µL of fluid.
12. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS + 0,5 % BSA.

Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 3 hours after lysis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.6 mg/mL

Buffer: The conjugate is provided in aqueous buffered solution containing protein stabilizer, and 0.09% sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store in the dark at 2-8 °C.

Date de péremption: 12 months

Détails sur CD52

Antigène: CD52

Autre désignation: CD52

Sujet: CD52 clone HI186 reacts with CD52, also known as CAMPATH-1, a protein of 6,6 kDa. It is expressed on male reproductive tract (epididymis, seminal vesicle), thymocytes, eosinophils, lymphocytes, red cells, monocytes and macrophages, but not on plasma cells, platelets or erythrocytes. CD52 plays a role in carrying and orienting carbohydrate
This molecule is expressed on lymphoid malignancies at variable levels and on epithelial cells of the epididymis and seminal vesicles. Its functional role is not well known but reports indicate that CD52 is a good target for complement-mediated cell lysis and antibody-mediated cellular cytotoxicity. Antibodies to CD52 have been studied in lymphocyte depletion experiments. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
Synonyme: CDw52, Cambridge pathology 1 antigen, CAMPATH-1 antigen, Epididymal secretory, protein E5, Human epididymis-specific protein 5, He5